Challanges File Issue while renew of Certificate

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My domain is: mail.blgindia.in

I ran this command: sudo certbot -v renew

It produced this output:

"
Saving debug log to /var/log/letsencrypt/letsencrypt.log


Processing /etc/letsencrypt/renewal/mail.blgindia.in.conf


Certificate is due for renewal, auto-renewing...
Plugins selected: Authenticator webroot, Installer None
Renewing an existing certificate for mail.blgindia.in
Performing the following challenges:
http-01 challenge for mail.blgindia.in
Using the webroot path /var/www/html for all unmatched domains.
Waiting for verification...
Challenge failed for domain mail.blgindia.in
http-01 challenge for mail.blgindia.in

Certbot failed to authenticate some domains (authenticator: webroot). The Certificate Authority reported these problems:
Domain: mail.blgindia.in
Type: unauthorized
Detail: 65.20.79.94: Invalid response from https://mail.blgindia.in/.well-known/acme-challenge/ccSBRrq5C1nZMGi9han0P7H1L6TTHVYKJSjMXCtvT1U: 404

Hint: The Certificate Authority failed to download the temporary challenge files created by Certbot. Ensure that the listed domains serve their content from the provided --webroot-path/-w and that files created there can be downloaded from the internet.

Cleaning up challenges
Failed to renew certificate mail.blgindia.in with error: Some challenges have failed.


All renewals failed. The following certificates could not be renewed:
/etc/letsencrypt/live/mail.blgindia.in/fullchain.pem (failure)


1 renew failure(s), 0 parse failure(s)
Ask for help or search for solutions at https://community.letsencrypt.org. See the logfile /var/log/letsencrypt/letsencrypt.log or re-run Certbot with -v for more details.
"

My web server is (include version): Nginx

The operating system my web server runs on is (include version): Ubuntu 22.04.3 LTS x86_64

My hosting provider, if applicable, is: Vultr

I can login to a root shell on my machine (yes or no, or I don't know): Yes

I'm using a control panel to manage my site (no, or provide the name and version of the control panel): No

The version of my client is (e.g. output of certbot --version or certbot-auto --version if you're using Certbot): Certbot 1.21.0

Do you know what the webroot for mail.blgindia.in is?

Maybe , yes!
When checked the configuration file it was like this

# renew_before_expiry = 30 days 
version = 1.21.0
archive_dir = /etc/letsencrypt/archive/mail.blgindia.in
archive_dir = /etc/letsencrypt/live/mail.blgindia.in/cert.pem
archive_dir = /etc/letsencrypt/live/mail.blgindia.in/privkey.pem
chain = /etc/letsencrypt/live/mail.blgindia.in/chain.pem
fullchain = /etc/letsencrypt/live/mail.blgindia.in/fullchain.pem

# Option used in the renewal process 
[renewalparams]
account = d4ce09f4c226b8944ef0e70240f1187f
authenticator = webroot
webroot_path = /var/www/html,
server = https://acme-v02.api.letsencrypt.org/directory
[[webroot_map]]
mail.blgindia.in = /var/www/html

The " character on the end should not be in that file.

Did you manually update the renewal conf file at any time?

The question relates to the root found in the web server.
[we know that is what certbot is looking for]

Show:
nginx -T

nope I didnt