Maybe not. But, be sure to check your EC2 Security Group and that it allows inbound requests on port 443. Also check any VPC ACL Rules you may have set or AWS firewall rules.
How did you get the cert? Because I can't reach your domain using port 80 and HTTP. Using an HTTP Challenge is the most common method. I'll guess you used a DNS Challenge for your Route53 domain. Is that right?
curl -i -m8 http://stream.isenradio.com
curl: (7) Failed to connect to stream.isenradio.com port 80 after 178 ms:
Connection refused
curl -i -m8 https://stream.isenradio.com
curl: (28) Operation timed out after 8001 milliseconds with 0 bytes received