Certificates signed by ISRG Root X1 aren't enabled for client authentication on Windows

Let's Encrypt certificates are not really Client Authentication certs as this isn't in their intended purpose as far as I know. See also: Can I create client certificates for a received LetsEncrypt certificate? - #2 by stevenzhu

You would generally issue Client Certificates from your own in-house Certificate Authority (such as Active Directory Certificate Services, or another self-hosted CA like smallstep-ca).

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