BINGO!
It seems the University is using Palo Alto firewalls and they need to be instructed to allow the LE challenge requests to reach your web server.
Below we can see the first request [from my system] reaches your server.
The second request [simulating the LE request] does not have the same response.
It is interrupted by the firewall:
#1
curl -Ii dna.engr.latech.edu/.well-known/acme-challenge/Test_File-1234
HTTP/1.1 301 Moved Permanently
Date: Mon, 02 Jan 2023 02:28:04 GMT
Server: Apache
Location: https://dna.engr.latech.edu/.well-known/acme-challenge/Test_File-1234
Content-Type: text/html; charset=iso-8859-1
#2
curl -Ii dna.engr.latech.edu/.well-known/acme-challenge/Test_File-1234 -A "Mozilla/5.0 (compatible; Let's Encrypt validation server; +https://www.letsencrypt.org)"
curl: (52) Empty reply from server